Absolute Basics of HPLC & LC-MS
What will you learn on this course?
Your Complete Beginner’s Guide to High Performance Liquid Chromatography & Mass Spectrometry
Learn the fundamentals of High Performance Liquid Chromatography (HPLC) and Liquid Chromatography ‑ Mass Spectrometry (LC-MS) with this beginner‑friendly 1-day theory course. Build confidence in chromatography, mass spectrometry and applying your newly learned knowledge to real‑world applications. This HPLC and LC-MS course is Royal Society of Chemistry approved training for purposes of Continuing Professional Development (CPD).
Whether you’re stepping into analytical chemistry for the first time or looking to strengthen your technical expertise, this 1-day essentials training gives you the confidence and clarity you need to understand, and start using, HPLC and LC-MS. These foremost analytical chemistry techniques are used to separate, identify and quantify components in complex mixtures and therefore contribute in most labs. This course breaks complex instrumentation and techniques into easy-to-understand concepts, helping you build skills that you can apply in the lab.
Why Join the Absolute Basics of HPLC and LC-MS?
On completion, you will be able to understand what HPLC and LC-MS instruments do, how they work, and why they are one of the most used analytical chemistry tools today. This course gives you:
- Strong foundational knowledge with no assumed experience
- Clear explanations and examples of chromatography and mass spectrometry principles
- Confidence to start to interpret data and understand what it means
- Insights into real applications across multiple industries using animated applications
- Better understanding of the instrumentation with instrument parts and consumables to see.
- A meaningful certificate for your CPD training, whether you’re in a technical role, a manager or an adjacent industry
This is your opportunity to understand two of the most widely used analytical techniques in modern science.
What You Will Learn on this HPLC and LC-MS
A complete beginner‑friendly introduction focusing on the what, how, why, where and when of HPLC and LC‑MS, including:
What HPLC and LC‑MS actually are
- What is liquid chromatography?
- What is mass spectrometry?
- What is specific to HPLC and its hyphenated technique LC‑MS
How HPLC and LC-MS work
- The core principles behind chromatographic separation
- How mass spectrometers detect and measure analytes
- Instrument components, functions and workflows
Why we use HPLC & LC‑MS
- The problems they solve
- The answers and insights they provide
- Basic qualitative and quantitative data analysis
Where HPLC and LC-MS are applied
- Suitability of types of samples and analytes
- Real examples across pharmaceuticals, environmental science, food testing, forensics, clinical labs, petrochemicals and more
Learning outcomes
By the end of the course, you will:
- Understand the different types of chromatography
- Clearly define LC, HPLC, MS and LC‑MS, and appreciate the differences
- Know the key components of the liquid chromatograph and mass spectrometer
- Comprehend the principles behind data generation and data analysis
- Be familiar with common sampling and injection techniques
- Recognise how HPLC and LC‑MS are used across diverse industries
- Feel confident discussing instrumentation, workflows and results
- Understand what’s next in the your path of progression for HPLC and LC-MS
- Receive a acronym glossary on HPLC and LC-MS
This course gives you the language and understanding needed to work effectively with chromatographic systems, even if you’re not the person pressing the buttons.
Who is this course for?
This training is designed for anyone who needs a solid grounding in HPLC or LC‑MS, including:
- Complete beginners to chromatography or mass spectrometry
- Analysts & technicians wanting a clear, structured refresher
- Laboratory or line managers overseeing instruments
- Data analysts and report writers who interpret results but don’t use the instruments
- Service and consultant engineers who need a chemistry-focused viewpoint
- Sales, marketing or commercial teams working with HPLC and LC‑MS systems, consumables or laboratory services
If HPLC or LC‑MS touches your work in any way, this well-planned course delivered by experts in the field equips you to understand the science behind the technique with confidence.
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